Crown-X: A Novel Menstrual Stem Cell-Based Therapy for Hair Regeneration in Male Pattern Baldness

Male pattern baldness (MPB) is a prevalent condition affecting approximately 50% of men by the age of 50, significantly impacting psychological well-being and quality of life. Current hair regrowth treatments suffer from limited efficacy, high costs, and time-consuming processes. This study investigates the potential of Crown-X, a novel hair growth formulation infused with Menstrual Stem Cells (MenSCs), in promoting hair follicle regeneration. Molecular docking analysis was performed to evaluate the binding affinity of estradiol (a key component of MenSCs) with keratin (AlphaFold ID: Q9BYQ0) using AutoDock Vina. The docking results indicated a binding affinity of -5.5 kcal/mol, suggesting a stable interaction between estradiol and keratin. To further assess the efficacy of Crown-X, in vitro studies were conducted on human dermal papilla cells (hDPCs) to evaluate its proliferation-inducing effects.The results demonstrated a significant increase in hDPC proliferation in a dose-dependent manner, with the highest proliferation (63%) observed at 0.1% concentration after 72 hours of treatment. A saturation effect was noted beyond this concentration, indicating an optimal therapeutic threshold. These findings suggest that Crown-X effectively stimulates hair follicle cells, offering a promising solution for MPB treatment. In conclusion, the study provides strong evidence supporting the regenerative potential of Crown-X as an innovative and non-invasive hair restoration therapy. Future clinical trials will be essential to validate these findings and optimize formulation efficacy for widespread application in MPB treatment.