The Role Of Micro-RNA200-c In Implantatıon Of Embryo To Endometriyum

The objective of this study was to examine the impact of microRNA-200c on embryo implantation and the expressions of α5β3, LIF, IL-6, CD105 (endogolin), and VEGF, which have a role in implantation. The experimental model comprised 30 female and 4 male rats. The rats were divided into two groups: the first group comprised non-pregnant rats, while the second group comprised pregnant rats. The pregnant rats were then separated into two groups: an experimental pregnat group and a control pregnant group. In the experimental group, rats were administered a microRNA200-c mimic solution (50 µl of 0.9% sodium chloride) to the right uterine horn, while the left horn received an injection of 0.9% sodium chloride. The procedure was performed on day 3 of the pregnancy. The rats in the control group were not administered any treatment. On days 6 and 9 of gestation, the uteri were removed by cervical dislocation, after which the number of embryos was counted. The effects of α5β3, LIF, IL-6, CD105 (Endogolin), IL-6, CD105 (Endogolin) and VEGF proteins in the uterine tissues of the experimental pregnat groups were analysed using immunohistochemistry. MiRNA-200c reduced the number of implanted embryos by suppressing the expression of α5β3 integrin, LIF, IL-6, CD105 (endogolin), and VEGF. Based on these results, it can be concluded that mimic treatment may interfere with the process of implantation and early pregnancy by reducing the expression levels of In addition, integrin α5β3, LIF, IL-6, CD105 (Endogolin), VEGF.