Research on bacterial flora and antibiotic sensitivity of diabetic foot wound treated with antibiotic bone cement

Background Diabetic foot is one of the serious complications of diabetes mellitus, and diabetic foot wound infection is often fatal to patients. As a relatively new method, antibiotic bone cement treatment of diabetic foot is beneficial to many patients with diabetic foot. The aim of this study is to analyze the composition of diabetic foot microbiota and to screen antibiotics with better efficacy to add to bone cement for the treatment of foot infection wounds. Methods We collected the exudates from diabetic foot wounds of patients for bacterial culture and drug sensitivity test, and classified and analyzed the composition of bacterial flora. For drug susceptibility test results, we first screened the antibiotics by using the Boruta algorithm prediction model and then performed sensitivity analysis based on age and gender factors. Results According to this study, age was a significant factor in the cumulative sensitivity of bacteria to antibiotics added with bone cement. In the bacterial flora of diabetic foot wounds, positive bacteria accounted for a relatively high proportion, and the main species was Staphylococcus aureus. Based on the analysis, the better choices of antibiotics were Gentamicin(GEN) and Tobramycin(TOB) for negative bacteria and Moxifloxacin(MFX), Ampicillin(AMP) and Quinupristin-dalfopristin(QDA) for positive bacteria. Conclusions In the bacterial flora of diabetic foot wounds, positive bacteria accounted for a relatively high proportion, and the main species was Staphylococcus aureus. Based on our findings, it is advised that the diabetic population who requires bone cement antibiotic treatment be treated according to age, with GEN and TOB being recommended for negative bacteria and MFX, AMP, and QDA for positive bacteria.