A surface-imprinting lanthanide fluorescent hybrid probe on the SiO2 microspheres for the detection of the enrofloxacin

Enrofloxacin (ENFX), being an antibiotic of notable distinction, serves as a specialized therapeutic agent for the mitigation of animal afflictions. However, the potential ingestion of enrofloxacin through the consumption of animal-derived sustenance can give rise to a multitude of concerns such as toxicity, drug resistance, and allergic reactions. Therefore, it becomes imperative to embrace measures that expedite the quantification of enrofloxacin levels, ensuring timely detection and assessment. Herein, a surface-imprinted polymer SiO ₂ @Eu(DBM) ₃ phen/SMIP was synthesized using ENFX as a template molecule, methacrylic acid as a functional monomer, and Tris(dibenzoyl methane)(1,10-phenanthroline)europium(III) as a fluorescent moiety. SiO ₂ @Eu(DBM) ₃ phen/SMIP performs high selectivity and the low detection limit toward enrofloxacin. The specificity assay revealed its remarkable selectivity for four other fluoroquinolone antibiotics, while the rare-earth complexes displayed exceptional chemical stability, showing no significant interference from metal ions or reactive oxygen species. The results indicate that it is feasible to use SiO ₂ @Eu(DBM) ₃ phen/SMIP for the detection of ENFX as well as effective adsorption. This study investigates the self-assembly of rare-earth complexes on silica using suggestive blotting technique, which provides a new solution for the subsequent development of surface blotting technique.