Removal of bacterial contaminants in laboratory conditions using glass containers impregnated with Ag nanoparticles

Background Nanotechnology can be very beneficial and critical to make a significant impact on sciences such as health and medical purposes. Of these, nanoparticles have an important role and there are many attempts to apply them in diagnosis, disease treatment, gene therapy, dentistry, oncology, the aesthetics industry, drug delivery, and therapeutics due to their importance. However, it seems in the health field and disease prevention, we need more studies for applying the nanoparticles. So, the aim of this study is the evaluation of AgNP nanoparticles and antimicrobial activity against bacteria as self-cleaning properties of glass in a laboratory model. Methods In this study, we designed laboratory glass coated with Ag nanoparticles (AgNPs) to delete the bacterial contaminants to maintain the health of laboratory personnel. Ag nanoparticles were synthesized by chemical approach and examined by using Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) to determine their size, structure, and shape. Also, Fourier Transform Infrared Spectroscopy (FTIR) was performed to identify organic, polymeric, and, in some cases, inorganic materials and observe chemical properties. Ag NPs were deposited on the glass by dip coater apparatus and finally, the antimicrobial properties of Ag NPs were evaluated by agar well diffusion and colony count methods. Result Based on TEM and DLS's results the synthesized AgNPs are spherical and their sizes are between 50–100 nm. However, the most synthesized particles are 68.9 nm. The antibacterial tests showed that glass-coated AgNP nanoparticles had good antibacterial activity. Conclusion The AgNPs can prevent the colonization of bacteria on surfaces coated with the AgNPs and even more can inhibit their growth. Consequently, AgNP nanoparticles can be utilized to prevent contamination of equipment by bacteria. These glass, with their AgNPs or AgNPs / MPTM coating, have self-cleaning properties and prevent contamination or survival of microbes on their surfaces.