Preparation of magnetic dendrimer and immobilization of laccase for dye removal

  1. Tianwen Lu
  2. Yixuan Dai
  3. Yue Shi
  4. Xiaojun Wang
  5. Yinan Hong
  6. Hong Pan
  7. Zhansheng Wu
Read the full article See related articles

Listed in

This article is not in any list yet, why not save it to one of your lists.
Log in to save this article

Abstract

Laccase is a kind of green biocatalyst which is widely distributed in nature. However, the application of free laccase was greatly limited due to the poor stability and low utilization. In order to prepare a carrier with abundant immobilized sites, magnetic dendrimer (MPG-x) grafted by PAMAM was constructed using amino-functionalized Fe 3 O 4 as divergent nucleus in this study. Then the immobilized laccase MPG-2@GA-Lac and MPG-2@Cu 2+ -Lac were prepared by glutaraldehyde crosslinking and copper chelating adsorption, respectively. The results showed that the two immobilized laccases showed better comprehensive stability than free laccase. Moreover, the activation of Cu 2+ in the active center of laccase made MPG-2@Cu 2+ -Lac exhibit better stability. MPG-2@GA-Lac and MPG-2@Cu 2+ -Lac retained 74.4% and 108.1% of the initial free laccase activity, respectively. Compared with MPG-2@GA-Lac, MPG-2@Cu 2+ -Lac achieved better methyl orange (MO) removal over a wider pH and temperature range. This study is expected to further expand the application of laccase in the degradation of dye wastewater.

Article activity feed

  1. Version published to 10.21203/rs.3.rs-6529585/v1 on Research Square