In Vitro Expansion of Dendritic Cells Loaded with Placental Peptides for the Generation of Antigen-Specific T Cells and Their Antitumor Activity
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The study investigates the use of expanded dendritic cells (DCs) loaded with placental peptides for generating antigen-specific T cells capable of inducing potent antitumor activity against various tumor cell lines, including glioblastoma (U87MG), Neuroblastoma(SH-SYSY), and breast cancer (MCF-7). Through in vitro assays, we demonstrate the successful expansion of DCs in vitro, their maturation upon loading with placental peptides, and the subsequent activation of T cells. The cytotoxicity of antigen-specific T (AST) cells was evaluated, showing strong tumor cell killing against all three cancer cell lines. Our results indicate that placental peptides could be developed as novel antigens for DC-based cancer immunotherapy, offering a promising approach to targeted cancer treatment.
