Promotion of Pathological Cardiac Remodeling by Excessive Mitochondrial Fission in Sedentary Lifestyle-Associated Myocardial Infarction

A sedentary lifestyle such as prolonged periods of sitting is one of the most important modifiable risk factors for morbidity and mortality in myocardial infarction (MI). Recently, mitochondrial dynamics (fusion and fission) have gained considerable attention as imbalanced dynamics may play central roles in various organ injuries, including MI. This study aimed to elucidate whether imbalanced mitochondrial dynamics of cardiomyocytes are involved in sedentary lifestyle-associated MI using mouse models. An MI model created by permanent coronary artery ligation showed a decreased survival rate, and the hearts of mice developed cardiac necrosis in the apex, cardiomyocyte hypertrophy, reduced ejection fraction, inflammation, and fibrosis. Ultrastructural analysis revealed increased mitochondrial fission, abnormal cardiac remodeling such as sarcomere disruption, and increased mRNA expression of cardiac injury and mitochondrial fission markers. Compared to the simple MI model, the combined sedentary lifestyle model created by narrow cage breeding and the MI model showed a further decrease in survival rate, cardiac hypertrophy, increased mitochondrial fission in cardiomyocytes, and myofibroblast activation with cardiac fibrosis. These findings suggest that mitochondrial fission could be involved in sedentary lifestyle-associated MI via abnormal crosstalk between cardiomyocytes and fibroblasts. Our study highlights the importance of developing novel mitochondrial-dynamics-rebalancing treatments in patients with MI.