Isolation and characterization of pathogens associated with Muga silkworms using molecular tools in Sivasagar and Jorhat districts of Assam

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Abstract

The study, “Isolation and characterization of pathogens associated with Muga Silkworms using molecular tools in Sivsagar and Jorhat districts of Assam”, was conducted from 2021 to 2024 at Assam Agricultural University, Jorhat. The findings reveal significant threats to the Muga silk industry due to bacterial flacherie disease, predominantly caused by Pseudomonas spp. and Bacillus subtilis . Infected silkworms exhibited symptoms such as sluggish movement, fluid vomiting, body darkening, and larval hanging. Phylogenetic analysis confirmed that the MK3 ( Pseudomonas ) and MT4 ( Bacillus ) isolates are closely related to global strains, with pathogenicity tests demonstrating high virulence. The study emphasizes the need for effective disease management strategies, including genetic improvement of disease-resistant silkworms and host plants, genome sequencing, and sustainable disease control measures. Educating farmers, fostering international collaboration, and implementing sustainable practices are crucial for safeguarding Muga silk production and ensuring the industry's long-term viability.

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