Green formulated silver nanoparticles for attenuating sepsis- induced cardiac injury by suppressing inflammation

Sepsis-induced myocardial dysfunction, known as septic cardiomyopathy, is a potentially fatal illness marked by a systemic inflammatory response to infection; effective therapeutic methods are still challenging to come by. This study investigated the effects of green synthesized silver nanoparticles (AgNPs) on oxidative stress in sepsis-induced cardiac injury. Different informative characterization techniques, including UV-Vis spectroscopy, DLS, and TEM techniques, confirmed the environmentally synthesized AgNPs have a spherical morphology, with a hydrodynamic size of 218 nm, polydispersity index of 0.24, and a zeta potential of -24 mV. XRD and EDX analysis showed that the AgNPs were composed of Ag, C, and O with a face-centered cubic crystalline structure. The in vitro studies revealed the potent antioxidant activities of AgNPs against DPPH, ABTS, Hydroxyl radical (⋅OH), and Superoxide anion radical (O2⋅ ⁻ ). The biocompatibility of AgNPs was evaluated using MTT assay and hemolysis assay. The animal studies on the LPS-induced murine sepsis model showed that AgNPs exhibited promising cardioprotective effects via attenuating oxidative stress and inflammation and maintaining mitochondrial homeostasis. The study concluded that AgNPs improve redox balance and attenuate nitric oxide production, indicating potential protective effects. However, further research is needed to understand their impact on sepsis-related inflammatory responses better.