Bioactivity and GC-MS Profile of Extract from Static Cultures of Fungi Isolated from the Leaf of Solanum lycopersicum

This research investigated bioactive compounds produced by endophytic fungi isolated from Solanum lycopersicum (tomato) leaves, with a focus on antimicrobial and antioxidant properties. The fungal extract exhibited significant antimicrobial activity against Staphylococcus aureus , Streptococcus pyogenes , Enterococcus faecalis , Klebsiella pneumoniae and Candida albicans . The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values were 0.8 mg/mL, demonstrating the extract's potency, particularly against E. faecalis . The extract also showed antioxidant activity, with a 34.9% inhibition rate. TLC analysis, using a hexane: ethyl acetate: acetic acid (25:25:3) solvent system, identified two distinct spots (spots A and B) with Rf values of 0.50 and 0.80, respectively. Visualisation with p -Anisaldehyde revealed pink and deep blue colours, confirming the presence of various bioactive compounds. This research highlights the potential of endophytic fungi as valuable sources of antimicrobial and antioxidant agents, with significant implications for pharmaceuticals and public health.