A chromosome-scale reference genome of Moroccan Argane (Argania spinosa)

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Abstract

The Argane tree ( Argania spinosa ), a Moroccan native species, has significant ecological and commercial value. To create a high-quality reference genome, we performed whole-genome sequencing with PacBio HiFi long reads and Hi-C data for chromosome scaffolding. The genome size was estimated in silico, and contamination was checked to assure data quality. The final assembly consists of 10 pseudochromosomes and 40 unplaced scaffolsd with a total length of ~ 662 Mb, a N50 of 80 Mb, and a 34.35% GC content. The BUSCO completeness test indicated 99.1% complete genes, indicating that the assembly was of excellent quality. In addition, a phylogenomic study was carried out using 750 BUSCO genes to assess A. spinosa's evolutionary position among similar species. This high-quality genome gives crucial information for conservation and genetic advancement. All sequencing data and assemblies are publicly available, ensuring reproducibility and further analyses.

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