Evaluating the Anti-Lung Cancer activity of Berberis vulgaris in several in-vitro methods

Background: Lung cancer, characterized by uncontrolled cell growth in lung tissue, poses a challenge to global health for its aggressive nature and poor prognosis. Despite advancements in medical research LC remains a leading cause of cancer-related deaths, highlighting the urgent need for innovative therapeutic strategies. This study investigates the effect of BV on the NSCLC, specifically LLC. Methods: This study investigated the anti-cancer properties of BV using in -vitro models- Anti-mitotic assay, Brine Shrimp Lethality Assay and MTT Assay. In anti-mitotic assay the inhibition of mitosis cell division along with the root no & root length were observed in Allium cepa . The BSL model was performed to observe the lethality of BSL .The MTT Assay further confirmed the cytotoxic effects on NCI-H460cell lines, showing a dose-dependent reduction in cell viability. Results: In the Anti-mitotic assay, the same root no & length in the treatment groups with proper inhibition of mitosis cell division, In the BRS model 100 % lethality rate in test drug groups, in the MTT assay decreased rate of cell viability in the group of Test drug were observed . Conclusion: The study demonstrates that BV exhibits its potential & promising anti-cancer properties by showing positive results in all models.