Aster peduncularis Wall. Ex. Nees: In- Vitro Antibacterial and Antileishmanial Activities of Essential oil from India

The study examined the antibacterial and antileishmanial properties of the essential oil extracted from Aster peduncularis Wall. Ex Nees. The antibacterial activity of this essential oil was studied using disk diffusion and broth microdilution methods on five human pathogen bacteria ( Salmonella typhimurium( MTCC No.3224), Pseudomonas aeruginosa ( MTCC No. 424), Bacillus subtilis ( MTCC No. 441), Escherichia coli ( MTCC No. 443), Klebsiella pneumoniae ( MTCC No. 3348) and six plant pathogenic bacteria ( Xanthomonas campestris( BB0006), Xanthomonas oryzae ( BH0007), Ralstonia solanacearum ( BI0012), Erwinia crysanthemi ( KUMSCC 328), Xanthomonas phaseoli (KUMSCC 327), Agrobacterium tumefaciens ( MTCC No. 609). The human bacterial strain P . aeruginosa was the most effective for oil (13.50 mm, MIC 75 µl/ml), followed by E . coli (13.05 mm, MIC 75 µl/ml), S . Typhimurium (10.40 mm, MIC 100 µl/ml), B . subtilis (10.20 mm, MIC 100 µl/ml) and K . pneumonia (10.20 mm, MIC 100 µl/ml). Among plant pathogens, X . phaseoli (18.50 mm, MIC 50 µl/ml) had the most potent antibacterial activity, followed by R . solanacearum (17.90 mm, MIC 50 µl/ml) and E . chrysanthem um (13.50 mm, MIC 75 µl/ml). We did the antileishmanial activity of essential oils with Leishmania donovani (Dd8) promastigotes and intracellular amastigotes. EO was safer on the J774.A1 macrophages and lethal to promastigotes and intracellular amastigotes at different concentrations (10, 50, and 100 µg/ml). These findings support the traditional uses of A. peduncularis and suggest its potential as a natural remedy for bacterial infections and leishmaniasis.