Anti-cancer efficacy of combination of vaccine-strain measles and mumps viruses against colorectal cancer in experiment

Objectives Evaluation of oncolytic efficacy of measles and mumps virus combination against colorectal cancer cells (HT-29) in vitro and the nude mouse xenograft model. Materials and methods MTT assay and flow cytometry assay were used to evaluate post-infection viable HT29 cells and apoptosis in vitro . 40 nude mice 6–8 week old age, were divided into 4 groups (10 mice/group) and formed the nude mouse xenograft model for assessing colorectal oncolytic efficacy in vivo . Results The viable cell and apoptotic cell death rates of the viral combination-treated combination group were lower (p < 0.05) and higher (p < 0.01) more than those of the single virus-infected groups, respectively. The tumor sizes, survival time and death rates of the viral combination-treated combination group significantly slowly increased (p < 0.001), was longer (p < 0.05) and lower (p < 0.05) more than those of the single virus-treated groups, respectively. Conclusions the measles and mumps combination virotherapy had the better synergistic anti-cancer efficacy against colorectal than the single virus-treated therapy cancer in vitro and in vivo .