Acute inflammation induced accelerated vascular remodeling by blood mononuclear cells in vitro
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The plasticity of blood mononuclear cells (MCs) and their role in vascular remodeling have been the focus of many studies; however, their in vitro differentiation efficiency remains poorly understood. Herein, we demonstrate that the inflammatory response accelerates the efficiency of MCs differentiation into endothelial-like cells through chemical cues in vitro . RT-PCR and RNA sequencing revealed that the differentiated cells exhibited upregulated pathways associated with vascular remodeling and regeneration. In contrast, MCs collected from normal blood showed a differentiation bias towards macrophages. Notably, under inflammatory conditions, most MCs transitioned into the CD14++/CD16+/CD163 + subset, which primarily contributed to vascular regeneration. This transition was triggered by inflammation, as confirmed by in vitro cytokine differentiation. These results imply that acute inflammation accelerates MCs-induced vascular remodeling.
