The full‑length genome sequence of a novel polymycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea

Here, a novel dsRNA virus belonging to the family Polymycoviridae was identified in phytopathogenic fungal strain B. dothidea ZM200473, and tentatively named “Botryosphaeria dothidea polymycovirus 2” (BdPmV2). The genome of BdPmV2 have five genomic dsRNAs, ranging from 1224 to 2407 bp, encoding five putative open reading frames (ORFs), of which ORF1 encodes a conserved RNA-dependent RNA polymerase (RdRp) consisting of 763 amino acids (aa) with a molecular mass of 84.03 kDa, ORF3 encodes a putative methyltransferase (Met) consisting of 627 amino acids (aa) with a molecular mass of 68.41 kDa, ORF4 encodes a P-A-S-rich protein behaving as coat protein (CP) consisting of 261 amino acids (aa) with a molecular mass of 27.60 kDa. ORF2 and ORF5 encode putative proteins with unknown functions which consisting of 697 amino acids (aa) with a molecular mass of 76.49 kDa and consisting of 323 amino acids (aa) with a molecular mass of 33.92 kDa, respectively. BLASTp analysis revealed that the RdRp protein of BdPmV2 had the highest similarity 56.52% to a virus previously identified as " RNA dependent RNA polymerase of Aspergillus fumigatus polymycovirus 1". Phylogenetic analysis based on the RdRp aa sequence indicated that BdPmV2 is a new member of the family Polymycoviridae .