A simple and convenient method for antimicrobial susceptibility testing of plant pathogenic bacteria.

Antimicrobial resistant (AMR) bacteria is the common threat in clinical, stockbreeding, aquaculture, and agricultural fields. To prepare for new AMR bacteria, we developed a simple and convenient method for antimicrobial susceptibility testing (AST) of plant pathogenic bacteria. The new recipe for media preparation and bacterial inoculation was proposed with understandable instructions, based on Goto and Takikawa (1984). Nutrient agar with 1% glucose were equivalent to the original medium. Inoculation using cotton swabs allow users to observe clear inhibition circles. The AST method for plant pathogenic bacteria was not standardized so far, but new method enables comparison and accumulation of data.