Evaluation of Stress Biomarker in Migraine and Tension Headache Patients Before and After Botulinum Toxin Injection

Migraine is a highly prevalent and disabling disorder. Because stress appears to be a prominent trigger of this condition. the aim of this study was to evaluate the therapeutic effects of botulinum toxin injection in migraine and tension headache patients and to estimate salivary cortisol, salivary alpha amylase in these groups of patients before and after treatment with botulinum toxin injection. A total of 45 subjects were divided into two groups 25 patients with chronic migraine and 20 patients with chronic tension headaches. The salivary biomarker (salivary cortisol, salivary alpha amylase) was detected by enzyme-linked immunosorbent assay (ELISA) kits. The result showed the increased statistically significant (p < 0.05) in salivary cortisol, and decreased statistically significant (p < 0.05) in salivary alpha amylase after intervention with botulinum toxin in both study groups (chronic migraine and chronic tension headaches). Salivary amylase decreased significantly after therapy in both groups (migraine and tension headache). Salivary cortisol increased significantly after therapy. In addition, Nausea and vomiting responded best to therapy, followed by orofacial discomfort.