Co-culture for improving the biosynthesis ability of Huperzine A

Huperzine A (HupA) is for treating Alzheimer's disease(AD) which was mainly extracted from the Huperzia serrata(HS) . Especially, (-)-HupA extracted from HS with high inhibitory activity of acetylcholinesterase are scarce and the chemical synthesis of (+)- HupA is high toxicity with low acetylcholinesterase inhibitory activity. In this work, Serratia marcescens HL1 was firstly found and isolated from HS , that was identified according to their morphological characteristics and nuclear 16SDNA sequences. It could biosynthesize HupA(BHA) of 32.976 ± 0.21 mg/L which was co-cultivated after with endophytic fungi Trichoderma harzianum NSW-V. Moreover, this bacteria with shorter fermentation time could form better purity and crystal structure with the same physicochemical properties compared to (-)-HupA isolated from HS (PHA) according to the results of NMR and molecular docking. Furthermore, this study explored new indications for HupA which indicated it could protect β-cells of pancreatic islets.