Amelioration of hepatic injury through oxidative stress management employing methanolic extract of Crepe- ginger (Cheilocostus speciosus (J. Koenig) C. Specht) flower

Background: Cheilocostus speciosus (J. Koenig) C. Specht, commonly known as “Crepe-ginger”, is a traditional plant with edible flowers utilized in folk medicine. This study employs crepe-ginger flowers to evaluate their role in boosting liver immunity, hepatoprotective actions through oxidative stress management. Methods: Cheilocostus speciosus flower’s methanolic extract (CSF-ME) was subjected to In-vitro anti-oxidant effects were evaluated using DPPH and ABTS and in-vivo by catalase (CAT) assays which ameliorated CCl ₄ -induced hepatic injury evident by histopathological analysis. The chemical assay was evaluated via phytochemical screening and GC-MS/MS analysis followed by in-silico studies. Results: The antioxidant assay DPPH (IC ₅₀ =179.36 µg/ml) and ABTS (IC ₅₀ = 198.27 µg/ml) showed remarkable scavenging activity. Hepatotoxicity experiments demonstrated that CSF-ME improved liver function by positively regulating AST, ALT, ALP, bilirubin, creatinine, LDL, CHO, TG, HDL, and catalase levels. Besides, histopathological analysis revealed normal hepatocyte integrity and microstructures after treatment. Besides, phytochemical screening revealed prospective phytochemical groups while GC-MS/MS analysis recognized forty compounds resulting in auspicious outcomes employing computer-aided studies. Conclusion: The findings indicated that the CSF-ME possesses promising hepatoprotective, and antioxidant prospects which demand further extensive research to develop novel lead compounds from this natural source.