Regulation of LncRNA DNAJC3-AS1/hsa-miR-576-5p/SSBP2 Axis in Gastric Cancer Migration

Background DNAJC3-AS1 overexpression drives cancer progression, particularly in gastric cancer (GC). This study delves into its role in GC, focusing on the regulation of miR-576-5p and its impact on cell apoptosis, migration, and proliferation. methods The expression levels of SSBP2, miR-576-5p, and DNAJC3-AS1 were measured in both adjacent non-tumor and GC tissues from patients with primary GC, as well as in a cell line. Apoptosis, cell cycle, migration, and cell proliferation were detected by flow cytometry (FCT), wound healing assay, and CCK-8, respectively. The dual luciferase reporter assay was applied to determine the binding relationship between hsa-miR-576-5p and DNAJC3-AS1 , as well as hsa-miR-576-5p and SSBP2 . Results Expression of DNAJC3-AS1 was significantly elevated in both cell lines and GC tissues. The direct binding of DNAJC3-AS1 and SSBP2 was validated, and hsa-miR-576-5p served as a sponge for SSBP2 in a competitive manner. Discussion By acting as a sponge for miR-576-5p and thereby modulating the expression of SSBP2, DNAJC3-AS1 was found to promote migration of HGC-27 cells, regulate cell proliferation, and inhibit apoptosis. The potential of DNAJC3-AS1 warrants further investigation for the early prognosis and detection of gastric cancer.