Investigation of Mycoplasma and Ureaplasma Species Using Molecular Method in Male Patients Suffering from Urethritis Symptoms
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Background: This study aimed to determine the frequency of Mycoplasma hominis , Mycoplasma genitalium , Ureaplasma urealyticum and Ureaplasma parvum in male patients suffering from urethritis symptoms. Methods: First-void urine and genital discharge samples were collected from 94 men. Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum and Ureaplasma parvum were investigated using a multiplex Polymerase Chain Reaction (PCR) method (BioGX Mycoplasma-Ureaplasma-OSR commercial multiplex PCR kit (BioGX, Netherlands)). Trichomonas vaginalis and Neisseria gonorrhoeae were investigated using microscopy and culture methods. In Mycoplasma genitalium positive samples, macrolide resistance was studied using the Macrolide-R/MG ELITe MGB Kit (ELITechGroup S.p.A., Italy) on the ELITe BeGenius® system Results: A total of 55 microorganisms were detected in 41.5% of the patients(n:39), [18 Ureaplasma urealyticum (33%), 14 Ureaplasma parvum (25%), 9 Neisseria gonorrhoeae (16%) , 8 Mycoplasma hominis (15%) and 6 Mycoplasma genitalium (11%) ] . Mycoplasma genitalium and Neisseria gonorrhoeae positivity was statistically higher in patients with more than three partners in the last 12 months, and patients with genital discharge had higher Ureaplasma urealyticum and Neisseria gonorrhoeae positivity (p<0.05). Additionally, a significant relationship was found between Neisseria gonorrhoeae positivity and genital itching and pain/discomfort during sexual intercourse symptoms (p<0.05). Macrolide resistance was detected in two of the six Mycoplasma genitalium positive samples (33.3%). Conclusions: The results of this study revealed that 41.5% of male patients suffering from urethritis symptoms had at least one from Ureaplasma urealyticum , Ureaplasma parvum , Neisseria gonorrhoeae , Mycoplasma hominis or Mycoplasma genitalium , respectively. The number of sexual partners is important in sexually transmitted diseases.
