Apoptosis Induction and S-Phase Cell Cycle Blockade in Human Lung Adenocarcinoma Cell Line (A549) by Chlorophytum comosum (Thunb.) Jaques

Methods The ethanolic roots (CCRE) and leaves (CCLE) extracts were interrogated for their apoptotic potential against human lung adenocarcinoma cell line (A549) using DNA fragmentation, Annexin V-FITC/PI staining apoptosis assay and cell cycle analysis using flow cytometry. Results Our results revealed significant DNA damage and apoptosis induced cell death in A549 cell line on treatment with active concentrations (40 µg/ml and 80 µg/ml) of the ethanolic extracts with S phase cell cycle arrest. Conclusions This is the first study demonstrating the apoptosis inducing potential of chemically characterized bioactive compounds present in ethanolic leaves and roots extracts from Chlorophytum comosum against non-small cell human lung adenocarcinoma cell line. The study concludes that Chlorophytum comosum can be a potential candidate for the natural bioactive compounds that can be isolated, characterized and clinically evaluated for the development of novel naturally derived anti-cancer drugs against lung cancer.