RP-HPLC Photodiode Array Based Method for Simultaneous Estimation of Aspirin and Pantoprazole Sodium

A specific, precise, accurate, robust, cost-effective and reproducible diode array detector based RP-HPLC method was developed and validated for quantitative analysis of Pantoprazole sodium and Aspirin in synthetic mixture. In RP-HPLC method drugs were separated by Inertsil C18 ODS (250 × 4.7mm, 5µm), utilizing a mobile phase buffer(0.05 m potassium dihydrogen phosphate pH 3 Adjusted by using 10% Orthophosphoric acid) : Acetonitrile (60 : 40%) at temperature(TEMP) 30º C, flow rate of 1 mL/min, injection volume 10 µL with UV detection at 230 nm. Aspirin and pantoprazole sodium were eluted with the retention time of 5.330 and 7.201 min respectively. The calibration plots were established over the concentration range of 4.05–121.5 µg/ml and 1–30 µg/ml for Aspirin and Pantoprazole Sodium with correlation coefficient 0.9997 and 0.9995 respectively. The method was found to comply all the validation parameter as per the ICH guideline, such as accuracy, precision, and linearity, Robustness, specificity. This method can be effectively employed for the day to day analysis of both drug.