Metaproteomics and metabonomics reveal the metabolic dysfunction of gut microbiota in Tibetan Minipigs in Atherosclerosis
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Atherosclerosis is fundamental in the development of cardiovascular disease. The unhealthy dietary habits, high fat and cholesterol intake could change the composition of gut microbes and metabolites which play a critical role in the development of atherosclerosis. However, few studies have systematically investigated the metabolism of gut microbes in atherosclerosis. In this study, we build an atherosclerosis model using the Tibetan minipigs, then we identified metabolites in the feces and serum, and explored the functions of the gut microbiota by metaproteomics. We found that, in the feces, multiple signal pathways showed obvious metabolic dysfunction that could influence the abundance of blood metabolic products. Several metabolites such as 3-dehydro-2-deoxyecdysone from cholesterol metabolism, leukotriene B4 from arachidonic acid metabolism, indole-3-acetate and 3-hydroxyanthranilate from tryptophan metabolism, 9,10-epoxyoctadecenoic acid from linoleic acid metabolism and 13(S)-HPOT from linolenic acid metabolism were significantly increased in the blood. These partially increasing metabolites were associated with inflammation that contributes the development of atherosclerosis. Our finding could provide novel clues for studying on the mechanism of arteriosclerosis.
