Anti-inflammatory response of Metformin Nanoparticles and/or low doses γ-Irradiation on Acute Pancreatitis in Rats via regulation of inflammatory cytokine

Repeated pancreatic insult inflammation leads to the development of pancreatitis. In these studies the evaluation of nano-formulated metformin drug with chitosan (Cs-Met NPs) and/or low-doses of γ-irradiation (IR) against acute pancreatitis (AP) induced by L-arginine in an animal model. Thirty adult of Albino rats were divided randomly into 5 groups. Normal control, L-Arginine treated (AP); AP + IR; AP + Cs-Met NPs and AP + IR + Cs-Met NPs. Histopathological studies of pancreatic and biochemical parameters (serum amylase and lipase levels, Random blood glucose (RBS), plasma insulin level, Insulin growth factor 1, tumor necrosis factor-alpha (TNF-α), IL-6, IL-8, IL-10, and CRP were measured in the pancreatitis model. AP induced by L-Arginine- treatment elevates the serum pancreatic amylase & lipase levels, significantly increases IL-6, IL-8, TNF-α, RBS, and CRP, and significantly decreases IL-10, insulin levels, and Insulin growth factor 1. Microscopic examination revealed loss of the pancreatic lobular architecture, marked fibrosis, acinar degeneration, inflammation, and marked oedema. All the serological parameters and the histopathological observations were markedly improved by Cs-Met NPs administration and/or low doses of γ-irradiation treatment. In conclusion, Cs-Met NPs and/or low doses of γ- irradiation have a therapeutic effect on acute pancreatitis model induced in rats.