Genome Survey of Sphallerocarpus gracilis Based on High-throughput Sequencing

Sphallerocarpus gracilis is a high-value medicinal and green health food product. The analysis of the genomic characteristic information of S. gracilis can lay a theoretical foundation for whole genome sequencing and molecular mechanism research of the biosynthesis of bioactive active ingredients. In this study, survey genome sequencing technology was employed to evaluate the genomic characteristics of S. gracilis using K-mer analysis, and smudgeplot analysis was used to evaluate its chromosome ploidy. The K-mer analysis results showed that the genome size of the sample was approximately 1,071 Mb, and the corrected genome size was 1,063 Mb. The heterozygosity rate, the proportion of repeat sequences, and GC content were determined 1.22%, 76.33%, and 35.70%, respectively. Based on the smudgeplot analysis, the maximum possible ploidy of the analyzed species was AB type, corresponding to a diploid plant. Blast analysis revealed S. gracilis to have a close relative relationship with Daucus carota (4.78%). In summary, the results indicate that the genome of S.gracilis is a complex and large genome with high heterozygosity and repetition and a large genome. This study provides a theoretical basis for future whole genome sequencing and related research.