Evaluation of antibiofilm and antimicrobial property of Cymbopogon citratus leaves extract fraction against Staphylococcus aureus Biofilm: in-vitro and Insilco approach

The aim of our work was to evaluated to the potential of different fractions of plant extract from C. citratus to impedance the S. aureus biofilm. The plant extract of C. citratus was obtained by Soxhlet apparatus and concentrated by rotary evaporator, here we identified the phytochemical bioactive compound by gas chromatography-mass spectrometry (GC-MS), and the antibiofilm activity of different fractions of the extract was analysed by measuring biofilm disruption and inhibition. The characterization of potential phytochemicals bioactive compound present in the extract was done using Fourier transform infrared (FT-IR) spectroscopy. Molecular docking study was performed with different bacterial components involve in the biofilm formation by interaction study between ligand and receptor binding of phytochemical substances. Different phytochemicals present into extract were quantified, showed that the highest total phenolic content is present in the aqueous extract (81.1 ± 0.0007 mg GAE/gm), while the highest total flavonoid content is present in the methanol extract (529 ± 7.0 mg QE/gm) and the highest total flavonoid content was present in the aqueous extract (74.6 ± 4.1 mg TAE/gm). The FT-IR spectra revealed various characteristic band functional groups such as amines, alcohol, fluoro compounds, phenol, alkane, alkene, and conjugated acid groups, conjugate aldehyde, Carboxylic acid was present in the extract. Bioactive compounds identified by GC-MS nearly 90 compounds were identified and 9 major potential phytochemical compounds were present in abundant in abundant in the extract. This study demonstrated that the phytochemicals present into the different extract possess antibiofilm activity by targeting different biofilm components.