A Green and Verified High-Performance Liquid Chromatographic Technique for the Concurrent Measurement of a Few Veterinary Drug Residues in Milk

Milk is a dietary item that is often consumed due to its high nutritional value. Milk containing residues of veterinarian medications is dangerous for human health and could negatively impact consumers. These leftovers are becoming more widely recognized as a developing environmental problem. This study developed and refined a chromatographic method for the simultaneous identification of specific veterinary medication residues in milk samples. The drugs that were selected were Sulfadimidine ( SDD ), Flunixin Meglumine ( FNM ), and Imidocarb Dipropionate ( IMD ). An ODS Hypersil C18 column with UV detection at 270 nm was used to perform the separation. A flow rate of 1 mL/min of 0.05 M phosphate buffer, pH 3: acetonitrile: methanol (55: 30: 15; by volume) used as the mobile phase. The target medicines were extracted from milk using a protein precipitation process that used methanol as a deproteinization agent prior to analysis. The suggested technique worked well for measuring the drug residues in milk samples that had been tampered with. In order to confirm that the suggested approach was an environmentally friendly and green analytical method, its greenness and environmental impact were also assessed.