Looking for a daptivegeographical SNPsin the Genus Epilobium

Epilobium is a genus of flowering plants in the family Onagraceae that contains approximately 200 species. This genus is distributed worldwide. It is most prevalent in the subarctic, temperate, and subAntarctic regions, whereas in the subtropics and tropics, Epilobium species are restricted to cool montane biomes such as the New Guinea Highlands. Many genetic and phylogenetic studies have been carried out on Epilobium , but none have focused on the geographical adaptation of SNPs. Therefore, we used ITS sequences of approximately 122 Epilobium taxa growing in different parts of the world and undertook computational analyses to identify discriminating SNPs among these species and to study their association with geographical variables. A combination of multivariate and Bayesian approaches, such as CCA, RDA, LFMM, and the skyline method, was used to identify several SNPs in these taxa with potential adaptation to geographical variables. Manhattan plot revealed many of these SNPs. are significantly associated with geographical variables? Genetic changes accompanied by local and geographical adaptation were illustrated using a skyline plot. These genetic changes did not occur under a molecular clock model of evolution, and probably under a positive selection pressure imposed in different geographical regions in which the plants grow.