Novel Bacteriophage Peptides Targeting Gamma-glutamyl transpeptidase (GGT) in Multidrug-Resistant Klebsiella Pneumoniae: A Promising in Silico Therapeutic Approach

Klebsiella pneumonia is a multi-drug resistance bacteria (MDR) play significant role in causing wide range of infections, including UTI, surgical site infections, septicemia and other bloodstream infections. The high rising ratio of MDR Klebsiella pneumonia is major public health concern and there is an urgent need to develop therapeutic strategies. Therefore, one of the hopeful and promising approaches is to use a bacteriophage peptide, which could specifically target the MDR bacteria and disrupt bacterial functions. The present study employs in silico analysis to screen and identify a new bacteriophage peptide which targets Gamma-glutamyl transpeptidase (GGT), a crucial protein in Klebsiella pneumoniae . The current study has identified and validated 20 important bacteriophage peptides against the GGT protein in Klebsiella pneumoniae using in silico analysis. The peptides show potential inhibitory effect on GG which could potentially decrease pathogen's viability. To further evaluate the efficacy and stability on this interaction we used comparative molecular dynamics simulation on four selected bacteriophage peptides complex with GGT. The results show the therapeutic potential of novel bacteriophage peptides, offering promising avenues for the development of targeted treatments against Klebsiella pneumoniae .