Hypoxia Mesenchymal Stem Cell Secretome Enhances IL-10 via STAT3 Pathway in a Rat PCOS Model

Background Polycystic ovary syndrome(PCOS) is a condition of chronic anovulation and hyperandrogenism which commonly causes infertility. PCOS is closely associated with chronic inflammation triggered by glucose and saturated fat, causing hyperandrogenism. PCOS has been proven to affect patient’s quality of life and cause infertility, so a better therapeutic approach is needed. The secretome of MSCs is able to suppress the secretion of pro-inflammatory cytokines and growth factors. Administration of secretome MSCs can inhibit the inflammatory response by increasing IL-10 expression and inhibiting androgen secretion in PCOS model mice. Objective: To prove the effect of administration of Hypoxic Mesenchymal Stem Cell Secretome on IL-10 and STAT3 gene expression in PCOS model mice. Method In vivo experimental research with a post-test only control group design. The total sample was 24 wistar female rats, divided into four groups: healthy, negative control (PCOS rats were injected with 0.9% NaCL), T1 (PCOS rats were given Secretome at a dose of 200 µl) and T2 (PCOS rats were given Secretome at 400 µl) and were given treatment for 33 days. IL-10 and STAT3 gene expression was tested using the One Way Anova test followed by the Post Hoc LSD test. Results This study showed that the expression of the IL-10 and STAT3 genes was significant different in the T2 group compared to the negative control and there was a significant difference in IL-10 gene expression in groups T2 and T1 compared to negative control. And also there were differences in the expression of the STAT3 gene in the T2 And T1 groups. Conclusion Administration of Hypoxic Mesenchymal Stem Cell Secretomes had an effect on increasing IL-10 and STAT3 gene expression in PCOS rat models.