In Vitro and In-Vivo Analysis of the Combined Extract of Tabernaemontana divaricata and Mangifera indica Leaves for Enhanced Anti-Inflammatory and Antioxidant Properties

Ethnopharmacological Relevance: Tabernaemontana divaricata ( T. divaricata ) and Mangifera indica ( M. indica ) are plants traditionally used in Ayurvedic medicine for their anti-inflammatory and antioxidant properties. T. divaricata is known for its analgesic and wound-healing effects, while M. indica is celebrated for its role in managing inflammation and oxidative stress. Aim of the Study: The study aimed to evaluate the synergistic effects of combined hydroalcoholic extracts from T. divaricata and M. indica leaves, focusing on their anti-inflammatory and antioxidant properties. Materials and Methods The leaves were collected, authenticated, and subjected to hydroalcoholic extraction. The extracts were then combined in various ratios and analyzed for phytochemical content. The antioxidant activity was evaluated using the DPPH assay, while anti-inflammatory effects were assessed through the carrageenan-induced rat paw edema model. Results The combined extracts exhibited significantly enhanced antioxidant and anti-inflammatory activities compared to the individual extracts. The most potent combination, a 2:1 ratio of M. indica to T. divaricata , showed the highest DPPH inhibition (94.37%) and maximum edema reduction in the animal model. Conclusion The combination of T. divaricata and M. indica extracts offers a synergistic therapeutic potential, enhancing both antioxidant and anti-inflammatory effects, which supports their traditional use and suggests potential for developing new natural therapeutics.