Investigation antibacterial activity of synthesized GO-SiO 2 nano hybrid against Escherichia coli as a bacterial index in water

Graphene oxide-silicon dioxide (GO-SiO2) nanocomposites are known for their potential antibacterial activity and unique characteristics. This study was aimed to investigate antibacterial activity of GO-SiO2 nanocomposites in various ratios. Scanning electron microscope (SEM) method was used to characterize morphological structure of GO-SiO2. Disk diffusion, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) method was used to investigation antibacterial activity in different nanoparticle ratios. The GO-SiO2 ratio of 1:1 exhibited the strongest inhibition zone against E. coli (20 mm), surpassing the individual antibacterial activity of GO and SiO2 alone. MIC and MBC tests provided valuable insights into the effectiveness of GO-SiO2 nano-hybrids against E. coli, with a MIC value of 6%, 2% and 2% for GO-SiO2 1:1 SiO2 and GO nanoparticles, respectively. MIC for GO-SiO2 in 1:2 and 2:1 ration >6% and >10% was resulted. Additionally, MBC of 12%, 4% and 4% was obtained for GO-SiO2 1:1 SiO2 and GO nanoparticles, respectively. This study successfully investigated the antibacterial properties of SiO2-GO nano-hybrids prepared through a novel imprinting route. The results demonstrate their potential as effective antibacterial agents, offering valuable insights for the development of advanced antibacterial materials.