Data mining and function analysis of a key ferroptosis target FANCD2 positively correlated with PD-L1 expression

Iron is required for the control of ferroptosis, a pattern of programmed cell death brought on by the buildup of reactive oxygen species and lipid peroxidation. Inducing ferroptosis may have great therapeutic promise for tumor cells, according to an increasing number of studies. However, it is unclear how ferroptosis works to treat lung cancer. For prognostic analysis of expression of 25 ferroptosis-related genes, consensus clustering analysis, LASSO model development, and association analysis with PD-L1, we gathered clinical and molecular data of LUAD patients from the TCGA database and the GEO database. We discovered that the ferroptosis gene FANCD2 had the best prognostic value of any gene based on the findings. Then, using multicolor immunofluorescence to identify the mutual regulatory link between FANCD2 and PD-L1 in LUAD tissues, we discovered that both of these genes were highly expressed and co-expressed in LUAD tissues. Finally, using four study axes—mutation, tumor microenvironment, immune infiltration, and pan-cancer—we discovered that FANCD2 may have a direct or indirect role in the aforementioned processes, influencing how well LUAD patients would fare. In conclusion, this research offers proof in favor of finding novel, potential immune targets for treatment as well as refining PD-L1 antibody immunotherapy for LUAD. In-depth analysis of the FANCD2 gene and the creation of associated medications may enhance prognosis and increase median survival time in LUAD patients.