Identification of pyroptosis-related biomarkers in venous thromboembolism

OBJECTIVE The study was designed with the aim of excavating diagnostic biomarkers of venous thromboembolism (VTE). METHODS The GSE19151 and GSE48000 datasets were subjected into this study. The pyroptosis-related genes (PRGs) were sourced from literature ¹ . Differential expression analysis and WGCNA were applied to identify differential genes related with Pyroptosis (DE-PRGs) in VTE. The possible functions of DE-PRGs were defined by means of enrichment analysis. The biomarkers related with pyroptosis in VTE were determined by plotting receiver operating characteristic (ROC) curve. The gene set enrichment analysis (GSEA) was employed to analyze the correlation between biomarkers and pathways. Finally, the quantificational Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) was proceeded to verify the expression level of the biomarkers in VTE. RESULTS A number of 52 DE-PRGs were identified by feat of differential expression analysis and WGCNA. A number of five biomarkers (RPL31, RPL34, RPL9, RPS27L and HINT1) were further screened by ROC curves. GSEA pointed to the linkage of five biomarkers to the ribosome proteins and oxidative phosphorylation signaling transduction, which may cause cell pyrodeath and trigger VTE through mitochondrial pathways. qRT-PCR manifested the expression levels of RPL31, RPL9 and HINT1 were all observably higher in VTE samples than in normal samples. CONCLUSION A number of five biomarkers, RPL31, RPL34, RPL9, RPS27L and HINT1, were identified as pyroptosis-related biomarkers in VTE, which provided a basis for understanding VTE pathogenesis and new insights into VTE diagnosis and treatment.