IL2RG’s Expression and its associated Long Non-Coding RNAs signature in ccRCC: Development, Validation, and Im-plications for Diagnosis,Prognosis,and Treatment

Background: Interleukin-2 Receptor Subunit Gamma (IL2RG) has been implicated in various cancers, but its role in clear cell renal cell carcinoma (ccRCC) remains unclear. This study aimed to explore IL2RG expression, its relationship with IL2RG -related lncRNAs (IRLs). Methods: qRT-PCR and IHC were used to assess IL2RG expression in ccRCC tissues and cell lines. Pearson correlation analysis identified IRLs related to IL2RG, and LASSO regression was applied to develop a prognostic model. We also conducted drug sensitivity analysis. Results: IL2RG was significantly upregulated in ccRCC tissues and correlated with advanced clinical stages (p<0.001). High IL2RG expression was linked to worse overall survival (OS), disease-specific survival (DSS), and progression-free interval (PFI) (p<0.05). A 6-IRLs signature was identified, and the resulting model accurately predicted survival outcomes. Immune-related pathways were enriched in high-risk patients, and drug sensitivity analysis indicated that high-risk patients were more responsive to sunitinib and temsirolimus. Conclusions: IL2RG and its related 6-IRLs are potential biomarkers for ccRCC progression. The 6-IRLs model provides a robust tool for predicting prognosis and guiding therapeutic decisions.