Next-Generation Sequencing Workflow Study Of SARS-Cov-2 Beta RBD in Complex with Human ACE2 With Biopython

In the present study, we focused on Next - Generation Sequencing analysis with Biopython to study the Beta SARS-CoV-2 variant RBD and Human ACE2 enzyme. The NGS workflow analysis includes computational tools MMDB, BioPython, BLAST, String, COBALT, InterPro, and PDB web server (PDBsum) for the present study. We employed a protein structural analysis to asses conserved domains which disrupt ACE2/SARS-CoV-2 Binding. Bio python was employed to automate the protein sequence for structure manipulation tasks. We performed MSA (Multiple sequence alignment) with COBALT and studied conserved residues that are essential for binding. Blast was used to search the homology and identify functionally relevant regions with a PDB web server that serves the purpose of visualization Protein domains and functions were classified with InterPro scan The findings of Interproscan of 8DF5 revealed peptidase and Collectrin –novel homolog of angiotensin-converting enzyme helps in the development of future therapeutic targets.