Using Microbial Inoculants for Enhancing Decomposition of Citywaste and Biohythane Production

Biohythane is a renewable energy source that carries great importance owing to its capacity to tackle energy security, environmental issues, and sustainable development goals. The current research focus on the the advantages of biohythane use as compared to the use of hydrogen and methane, as separate biofuels.The attention has been focused on the biohythane production from organic wastes, the most abundant organic substrates treated by anaerobic digestion, reporting the main milestones and the future trends. This research has primarily centered on the utilization of city waste to produce biohythane gases with the help of different consortia of microbes. Four treatments were used: T ₁ : Control, T ₂ : Organic waste +  Acidithiobacillus thioxidans/Acidithiobacillus ferroxidans ,T ₃ : Organic waste +  Methanosarcina thermophila / Methanobacterium beijingense and T ₄ : Organicwaste +  Acidithiobacillusthioxidans)/Acidithiobacillus ferroxidans)  +  Methanosarcina thermophila / Methanobacterium beijingense .The results indicated that the mean maximum value of CH ₄ was 74.2% in T ₄ where organic waste was used along with bacterial consortia followed by 64% in T ₂ .The highest value of H ₂ was 14.96% in T ₄ followed by 10.53% in T ₃ . The maximum value of H ₂ S was 63.3ppm in T ₄ followed by 54.66ppm in T ₂ . The highest value of CO was 850ppm in T ₂ followed by 680ppm in T ₃ . Sulfur-oxidizing bacteria, iron-oxidizing bacteria, and methane-producing bacteria were also used to enhance decomposition. Their cultures were obtained from local sources, and they were applied before the start of composting. It was concluded that the highest percentage of gases (CH ₄ ,H ₂ S, H ₂ and CO) were obtained from the anaerobic digestion where hydrogen-producing bacteria, methane-producing bacteria, and organic waste were applied together.