Transcriptome and metabolome changes uncover the potential mechanism of high-fat diet induced obesity under hypoxia condition

Aim : We aimed to investigated the key genes and metabolites in high-fat diet (HFD)-induced obesity model in hypoxia condition to uncover the potential mechanism. Method : Mice model in control, HFD, CIH, and HFDCIH were established through diet and hypoxia treatment. Then liver tissue samples were collected for transcriptome and metabolome analysis. Following, differential analyses were conducted to selected the unique genes and metabolites in HFD-induced obesity in hypoxia condition. Finally, functional analysis and correlation analysis were used to investigated the pathways and potential mechanism in obesity under hypoxia. Results : A total of 448 unique genes (like CD44, FCGR4, and COL6A1) and 36 unique metabolites were identified to be affected by obesity in hypoxia condition. The function of these key genes and metabolites indicated that these genes and metabolites may be related to metabolism-related function and pathways. The interaction between these genes and metabolites may be involved in Glutathione metabolism, Arachidonic acid metabolism, Histidine metabolism, Drug metabolism-cytochrome P450. Conclusion : The study uncovered the key genes and metabolites in obesity under hypoxia in mice model, and indicated the potential mechanism, providing indicator for the following focus.