Microbial-induced carbonate precipitation modulates microbial community composition in long-term lead-contaminated soil

Microbial induced carbonate precipitation (MICP) is known as a significant process for remediating heavy metals contaminated environment. At present, we have studied the effects of microbe induced carbonate precipitation on soil physical structure after remediation of lead (Pb) contamination. However, in the process of soil remediation, the addition of exogenous substances and bacterial solution to the structure of soil bacterial community and the relationship between soil fungi and non-biological factors remains unclear. In this study, a new type of urea decomposing bacteria was isolated and identified as Bacillus pasteurii . Its performances for immobilizing Pb in soil were systematically discussed at different treatment conditions. Results showed that effects of different biological treatments on basic physical and chemical properties of soil. Meanwhile, MICP reduced the available content of Pb in soil. By analyzing the diversity and composition of soil microbial communities, it was found that MICP reduced the diversity of soil microbial community and changed the composition of soil bacterial and fungal microbial communities due to the impact of bioremediation process on soil environmental factors. However, the functional composition of soil bacteria and fungi was not affected by MICP remediation, especially the functional composition of bacterial microbial community.