Fungi-metabolites production and networking analysis in saprophytes and pathogens fungi

Fungi are important parasitic microorganism of plants, which mainly include saprophytes and pathogens species. However, differences of mycotoxins production by different fungi still remain challenging. To address this bottleneck, saprophytes and pathogens Alternaria spp. are used for inoculation and metabolites production analysis are process via liquid chromatography- high resolution mass spectrometry (LC-HRMS). Characteristic neutral loss is present for modified mycotoxins screening and feature fragments which match chemical similarity are applied for annotation. The results revealed that Saprophytic Alternaria spp. are the dominant secondar metabolites producing fungi with extremely differences. Glucose conjugations, ALU-G, AME-G, and TeA-G, are identified as modified mycotoxins. In addition, molecular networking result reveals that metabolic pathway of Alternaria mycotoxins mainly include acetylation, dehydration, didehydration, dimethylamination, hydrogenation, isomerization, methylation, combination of methylation and hydroxylation, and combination of demethylation and dihydroxylation.