Research Influence Heat Shock on the Quality of Post Goat Oocytes Warming Through Evaluation Maturation Rate and Inspection Protein Heat Shock (HSP-70), Adenosine Triphosphate (ATP), Glutathione (GSH)

Local goat cultivation in Indonesia faces challenges due to imports of domestic goats, which threaten the genetic variation of local goats. To overcome this, veterinarians are directed to play a role in biotechnology, especially through the In Vitro Fertilization (IVF) method. This research is expected to show the identification of the three biomarkers, namely ATP, GSH, and HSP-70 from post maturation goat oocytes exposed to two different types of cryoprotectant, namely commercial cryoprotectant (Cryotech) and Ethylene glycol 30%+1M sucrose as well as comparing the quality of the goat oocytes after ELISA examination. This study used a completely randomized design with three groups of mature goat oocytes, consisting of one control group and two vitrification treatment groups with different cryoprotectants. Goat ovaries were collected from the Pegirian Surabaya slaughterhouse, then the oocytes were selected and matured in a CO2 incubator. The results showed that there was no significant difference in the maturation rate between the three groups of goat oocytes (control, Cryotech, and EG30%+1M Sucrose). However, there were significant differences in HSP70, GSH, and ATP levels. The EG30%+1M Sucrose group (Treatment 2) showed the lowest levels of HSP70 and GSH, but the highest levels of ATP compared to the other groups. This investigation demonstrates no significant difference in caprine oocyte maturation rates among the control and two cryoprotectant treatment groups. However, the EG30%+1M Sucrose treatment significantly altered HSP70, GSH, and ATP levels. Both cryoprotectants exhibited comparable efficacy in preserving oocyte quality during vitrification.