Exploration of m7G Modification Level and Immune Infiltration Characteristics in Patients with Primary Sclerosing Cholangitis

Primary Sclerosing Cholangitis (PSC) is closely associated with immune disorders influenced by both genetic and environmental factors. The role of post-translational modifications in PSC remains to be explored. In this study, we analyzed 20 differentially expressed m7G-related genes between 220 PSC patients and 320 healthy individuals. Through three clustering strategies, we identified that m7G Cluster A and Gene Cluster B exhibited similar biological processes and immune cell infiltration patterns, primarily involving macrophages, T lymphocytes, and pathways related to infection, tumor transformation, and myeloid-derived suppressor cells. Notably, these clusters were linked to higher m7G scores, indicating a potential relationship between m7G RNA and the pathogenesis and progression of PSC. Furthermore, we identified eight key m7G-related genes and constructed a nomogram model to predict the risk of PSC, providing insights into its underlying mechanisms and potential clinical applications.