Changes in the hematological profile, hepatic biomarkers, antioxidant capacity, and digestive functions of Sterlet sturgeon (Acipenser ruthenus) fed diet supplemented with dietary nucleotides
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This study investigated the effects of dietary nucleotides (NTs) on hematological indices, hepatic biomarkers, antioxidant capacity, digestive functions, and intestinal histomorphology of Sterlet sturgeon ( Acipenser ruthenus ). Over 10 weeks, five diets with varying NT levels (0 g/kg, 1.5 g/kg, 2.5 g/kg, 3.5 g/kg, and 5.0 g/kg) were fed to triplicate groups of fish (initial weight: 95.33 ± 1.23 g) in a flow-through system. Results indicated no significant differences in RBC, Hb, MCHC, HCT, and EOS among groups (p > 0.05). The highest WBC count was seen in fish on the 3.5 g/kg NT diet compared to the control (p < 0.05). Serum hepatic enzyme levels generally decreased with higher NT supplementation, although ALT increased significantly at the 5.0 g/kg level (p < 0.05). Antioxidant capacity improved in fish fed 0.25 and 0.35 g/kg NTs (p < 0.05), while serum MDA decreased with up to 3.5 g/kg NTs but increased at 5.0 g/kg (p < 0.05). Protease and amylase activity peaked in fish receiving 3.5 g/kg NTs (p < 0.05), with lipase activity highest at 2.5 g/kg NTs (p < 0.05). Intestinal histology revealed that fish on the 3.5 g/kg NT diet exhibited the greatest villi height and width, along with more goblet cells (p < 0.05). In conclusion, the present study indicated the optimum dietary level of NTs for positive effects on physiometabolic responses and intestine functions of the Sterlet sturgeon lies in the range of 2.2–3.6 g/kg.