First Report of Yambean Mosaic Virus Infection in Butterfly Pea (Clitoria ternatea) in Thailand

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Abstract

Butterfly pea ( Clitoria ternatea ) plants displaying mottling, vein banding, blistering, and wrinkling symptoms were observed in Chiang Mai province, Thailand, indicating potential potyvirus infection. Three symptomatic leaf samples, immature and mature seed samples, were collected for RNA extraction and tested via RT-PCR using degenerate primers targeting the coat protein (CP) gene of potyviruses. Four samples, excluding mature seeds, tested positive for potyvirus. Mechanical inoculation reproduced symptoms in butterfly pea, senna ( Senna occidentalis ), and yardlong bean ( Vigna unguiculata subsp. sesquipedalis ). Phylogenetic analysis confirmed infection by yambean mosaic virus (YBMV), marking the first report of YBMV in butterfly peas and in Thailand.

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