Complete genome sequence of Pseudomonas sp. HT11 isolated from broad bean (Vicia faba L.)

Bacterial strain HT11 was isolated from broad bean ( Vicia faba L.) and found to have strong antifungal activity against the fungus Botrytis fabiopsis (which causes red spot of broad bean). To better understand the secondary metabolites of the HT11 strain, its complete genome was sequenced and analyzed. It contained a single circular chromosome, with a length of 6,335,588 bp. The 16S rRNA gene comparison and the average nucleotide identity (ANI) analysis confirmed that the HT11 strain is a new Pseudomonas strain. The complete genome encoded 5,366 predicted open reading frames (ORFs), 66 tRNA genes, and 16 rRNA genes. The total length of the annotated genes accounted for 82.93% (5,254,103/6,335,588 bp) of the complete genome. The predicted functional ORFs were grouped into 24 Clusters of Orthologous Groups of proteins (COG) categories. Fourteen gene clusters were detected in the genome, which were involved in the synthesis of pyochelin, pyocyanin, viscosin, and tolaasin I/tolaasin F. There were also three gene clusters involved in the synthesis of unknown metabolites. These results lay the foundation for further research on the interactions between Pseudomonas sp. HT11 and the pathogenic fungus Botrytis fabiopsis .