Cloning and functional analysis of ClVND1, a member of the OsNAC7 subfamily in Chrysanthemum morifolium
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NAC transcription factors play important roles in plant growth and development. In this study, ClVND1 was cloned from the NAC transcription factor of Chrysanthemum lavandulifolium . Through bioinformatics analysis, the gene was found to belong to the OsNAC7 subfamily with an open reading frame (ORF) of 1164 bp and encoding 387 amino acids. Through paraffin sectioning and stress treatment, it was found that this gene could increase the thickness of the secondary cell wall of transgenic Arabidopsis stems and lead to the growth of more lateral roots to improve the tolerance of Arabidopsis seedlings to salt or low temperature. Moreover, the inflorescence elongation rate and height of wild-type Arabidopsis were greater than those of transgenic Arabidopsis , but the flowering period was later than that of transgenic Arabidopsis . These results indicate that ClVND1 may resist external stress by regulating root growth and can inhibit inflorescence growth and promote flowering. The purpose of this study was to improve stress resistance and promote the growth and development of C. morifolium by regulating the expression of ClVND1 , which is of great significance for the study of chrysanthemum resistance to abiotic stress and growth and development.