Chemical composition and nematicidal activity of wasabi(Eutrema japonicum) rhizome extracts against Meloidogyne enterolobii

Aims Eutrema japonicum a perennial herb belonging to the Eutrema genus in the Crucifer family. In recent years, numerous substances with notable pharmacological activities have been successfully isolated from E.japonicum . Despite significant advancements in related research, the efficacy of E.japonicum extracts against rhizome-knot nematodes remained unknown. Methods This study utilized extracts obtained from the rhizome of E.japonicum to evaluate their activity against J2 Meloidogyne enterolobii and single-egg hatching through a soaking method, demonstrating inhibition and killing activity against M.enterolobii . Results The results showed that the LC ₅₀ of E.japonicum extract on J2 were 44.633 mg/mL and 22.840 mg/mL at 24 h and 48 h post-treatment, respectively. The mortality rate of J2 reached 88.93% at 48h post-treatment when the concentration was 200 mg/mL, and the inhibition rate of single egg hatching reached 88.14%. This study conducted an analysis of the chemical composition of the ethanol extract of E.japonicum . we preliminarily screened out 10 organosulfur compounds and lipid compounds with insecticidal and antibacterial effects. Including Sec-butyl isothiocyanate and geraniol. Sec-butyl isothiocyanate and geraniol were further investigated for their nematicidal activity, demonstrating high efficacy against M.enterolobii .The results indicate that the extract of E.japonicum shows promise in inhibiting M.enterolobii . Conclusions These findings offer a scientific foundation and theoretical framework for utilizing E.japonicum as a potential raw material for the development of novel natural plant nematicides.